2

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Hack my probiotics experiment

Answered on August 19, 2014
Created September 02, 2012 at 4:55 AM

Hi, I have decided to test probiotics. I bought four different kinds of probiotics. I took two caplets/glass vials of each and dissolved them in a small plastic dish with milk. I left them sit overnight.

Milk was supposed to go sour, but it did not. In all four cases.

  1. What am I doing wrong if anything?
  2. Can all four be dead?
  3. Should I leave them out longer - for 24 hours maybe?

UPDATE on my experiment: two of them changed their consistency after 30 hours. One looks like really thick buttermilk and another one looks like thin buttermilk, but other two are just milk.

912822869eb7376f0fd4cc4afa72d9d7

(10)

on September 09, 2012
at 10:54 PM

Thanks for responding. Looks like I won't be trying out Nature's Way Pb anytime soon. I'm going to give this a go on my probiotics

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 04, 2012
at 06:24 AM

I used 1. Primadophilus bifidus by Nature's Way 2. and 3. Eastern European brands - Slovenian and some other 4. Armenian. The best brand was Armenian made locally (and the cheapest). The worst results - Slovenian brand and Nature's Way.

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 04, 2012
at 06:21 AM

It did not curdle it, it just made them look like thick and even buttermilk - there were no clumps. Is it curdled anyway?

A2c38be4c54c91a15071f82f14cac0b3

(12682)

on September 03, 2012
at 08:36 PM

It could mean a number of things, potentially the bacteria metabolized the lactose to acid and it curdled the milk.

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 03, 2012
at 06:42 AM

Two of them have changed the consistency after 30 hours. What does it mean?

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 02, 2012
at 06:06 PM

I can't do fermented foods... :( I get addicted and keep eating them like crazy. I am yet to try the drinks.

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 02, 2012
at 06:05 PM

Ok, I let it sit for 24 hours as you suggested. Then I tasted them all. One of them is a little bit sour. Not really sour, but with a sour hint. Does it mean it is a little bit alive? I don't have any pH strips and not sure where to get them. Can I just taste them with my tongue? Thanks for the answer!

Bb3d1772b28c02da2426e40dfcb533f5

(5381)

on September 02, 2012
at 06:27 AM

^ It does, altho diff bacteria eat different kinds of sugar and such. But then again, youd think at least one of the strains in a pill would eat lactose, as lactose is used often in SIBO challenge tests.

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 02, 2012
at 06:17 AM

Thank you. I needed someone to tell me this.

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 02, 2012
at 05:48 AM

Thanks! I am no expert either but I kind of think they are dead cultures. I will buy some sugar and add to them, just to see. I thought milk was supposed to have sugar in it (lactose).

3eca93d2e56dfcd768197dc5a50944f2

(11697)

on September 02, 2012
at 05:38 AM

Most probiotics come dead from the factory. Do some home-made goat kefir instead. It works, and it has trillions of different bacteria/yeasts, not a few billion (most of them dead) as in pills.

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 02, 2012
at 05:29 AM

Thanks! I am not expert either but I kind of think they all are dead. I will buy sugar and add some to them, just to see. I thought milk supposed to have sugar in it.

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4 Answers

2
A2c38be4c54c91a15071f82f14cac0b3

(12682)

on September 02, 2012
at 08:03 AM

Back in microbiology lab we used something called a litmus milk test to help identify bacteria, pretty much the same thing but with a pH indicator. If the probiotics you were using had mostly lactobaccilus (aka "milk bacterium") they should turn the milk sour (other probiotics may not thought). But we were cautioned that this test is prone to false negatives.

It's common to keep the test running longer, several days. Of course this opens up the possibility of contamination or residual milk bacteria skewing the results. You can set up control milk with no probiotics to help with this problem.

I personally wouldn't feel good about the results you got, but it's far from a damning test in my opinion. I'd try letting them go for 24 hours, perhaps more. Do you have any pH strips? Those can help you measure the acidity without the subjective measurement of tasting it.

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 02, 2012
at 06:05 PM

Ok, I let it sit for 24 hours as you suggested. Then I tasted them all. One of them is a little bit sour. Not really sour, but with a sour hint. Does it mean it is a little bit alive? I don't have any pH strips and not sure where to get them. Can I just taste them with my tongue? Thanks for the answer!

A2c38be4c54c91a15071f82f14cac0b3

(12682)

on September 03, 2012
at 08:36 PM

It could mean a number of things, potentially the bacteria metabolized the lactose to acid and it curdled the milk.

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 04, 2012
at 06:21 AM

It did not curdle it, it just made them look like thick and even buttermilk - there were no clumps. Is it curdled anyway?

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 03, 2012
at 06:42 AM

Two of them have changed the consistency after 30 hours. What does it mean?

1
912822869eb7376f0fd4cc4afa72d9d7

on September 04, 2012
at 12:24 AM

Which brands did you use? Which ones resulted in the change in consistency?

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 04, 2012
at 06:24 AM

I used 1. Primadophilus bifidus by Nature's Way 2. and 3. Eastern European brands - Slovenian and some other 4. Armenian. The best brand was Armenian made locally (and the cheapest). The worst results - Slovenian brand and Nature's Way.

912822869eb7376f0fd4cc4afa72d9d7

(10)

on September 09, 2012
at 10:54 PM

Thanks for responding. Looks like I won't be trying out Nature's Way Pb anytime soon. I'm going to give this a go on my probiotics

1
D7f404b29047b12e2c2f528934b7b80b

(133)

on September 02, 2012
at 06:37 AM

Yeah most are dead or at lower srength than stated. Just go for the fermented foods and drinks. More useful in their natural state.

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 02, 2012
at 06:06 PM

I can't do fermented foods... :( I get addicted and keep eating them like crazy. I am yet to try the drinks.

1
Bb3d1772b28c02da2426e40dfcb533f5

(5381)

on September 02, 2012
at 05:06 AM

Try putting some sucrose or glucose in their too maybe? And I have heard bacteria like warm & dark, so maybe put them in the hot water cupboard?

Also you could use water, which would cloud more obviously I think, rather than milk souring...

all just ideas, I am no expert on bacteria...

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 02, 2012
at 05:29 AM

Thanks! I am not expert either but I kind of think they all are dead. I will buy sugar and add some to them, just to see. I thought milk supposed to have sugar in it.

F5a0ddffcf9ef5beca864050f090a790

(15515)

on September 02, 2012
at 05:48 AM

Thanks! I am no expert either but I kind of think they are dead cultures. I will buy some sugar and add to them, just to see. I thought milk was supposed to have sugar in it (lactose).

Bb3d1772b28c02da2426e40dfcb533f5

(5381)

on September 02, 2012
at 06:27 AM

^ It does, altho diff bacteria eat different kinds of sugar and such. But then again, youd think at least one of the strains in a pill would eat lactose, as lactose is used often in SIBO challenge tests.

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